【摘要】 目的 :探讨中药复方益气解毒颗粒对人胚鼻咽上皮细胞转化的阻抑作用。方法 :二亚硝基哌 (DNP)诱导人胚鼻咽上皮细胞转化 ,同时用益气解毒颗粒作用于DNP诱导的鼻咽上皮细胞 ,共同培养 ,然后收集不同代数细胞 ,酶连免疫聚合酶链反应 (PCR -ELISA)和巢式聚合酶链反应 (Nested -RT -PCR)法检测实验细胞端粒酶活性和端粒酶RNA的表达。结果 :加益气解毒颗粒药液组鼻咽细胞形态明显好于盐水对照组 ,其端粒酶活性 (0 34± 0 19)也明显低于对照组 (0 73± 0 2 7) (P <0 0 5 )。结论 :益气解毒颗粒能够抑制鼻咽上皮细胞端粒酶的激活 ,进而阻断DNP诱导的细胞转化。更多还原

【Abstract】 To investigate the inhibitory effect of YIQIJIEDU Granule on the transformation of embryo nasopharyngeal epithelia induced by N,N’Dinitrosopiperazine(DNP) Methods:Nasopharyngeal epithelia from human embryo induced with water beg at 4～7 months were cultured together with DNP and YIQIJIEDU Granule for 24 h Then,the cells were collected for the observation of morphology and assays of telomerase activity and telomerase RNA expression with the techniques of PCR-ELISA and Nested-RT-RCP respectively Results:The cells incubated wity YIQIJIEDU Granule had much better morphology than those in the control group,and significantly decreased telomerase activity(0 34±0 19) compared with the control group (0 37± 0 27) Conclusion: YIQIJIEDU Granule can greatly prevent the normal nasopharyngeal epithelia from transforming induced by DNP through the inhibition of telomerase activation更多还原