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银染法与产物杂交法检测端粒扩增产物比较

Comparison between AgNO₃ staining and hybridization method used to detect telomerase activity in tumor cells

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【Author】 LEI Junxia 1) , CAO Mengde 1) ,ZHANG Ying 2) , QIN Dongchun 3) , YUE Baohong 3) , CHEN Zongde 1) , SU Di 1) , LIU Hongwei 1) 1) Department of Microbiology and Immunology, Henan Medical University, Zhengzhou 450052 2)On

【机构】河南医科大学微生物学与免疫学教研室；河南医科大学第一附属医院肿瘤科；河南医科大学第一附属医院检验科；河南医科大学微生物学与；

【摘要】目的 :选择简便、灵敏地检测端粒酶活性的方法。方法 :以HL 6 0细胞与慢性粒细胞白血病单个核细胞为检测对象 ,在端粒重复序列扩增技术 (telomericrepeatamplificationprotocol,TRAP)基础上 ,采用同步扩增产物杂交法和产物银染法检测其端粒酶活性。结果与结论 :产物杂交法操作简便 ,比产物银染法有更高的灵敏度 ,还可用于半定量。更多还原

【Abstract】 Aim: In order to search for rapid and sensitive method to detect telomerase activity in tumor cells. Methods: AgNO 3 staining and hybridization method based on telomerase repeat amplification protocol(TRAP) were concurrently used to detect telomerase activity in HL 60 strain and mononuclear cells of chronic myeloid leukemia. Results and conclusion: Hybridization method is more sensitive than AgNO 3 staining, moreover,it also can be used to relatively quantify telomerase activity.更多还原