【摘要】 目的 :对变性及非变性聚丙烯酰胺凝胶银染方法最适条件进行探讨和改良。方法 :采用PCR 聚丙烯酰胺电泳、硝酸银染色检测 15 9～ 196bp及 336bpDNA片段 ,并对不同显影液浓度与显色温度下的结果进行比较。结果 :DNA分子经改良银染法检测均清晰可见 ,对比良好。结论 :聚丙烯酰胺凝胶电泳后采用银染技术分析 ,方便快捷 ,易长期保存 ,并减小了对人及环境的毒害作用更多还原

【Abstract】 Aim:To study and modify the optimal methods of silver staining of denaturing and non denaturing polyacrylamide gels.Methods:polymerase chain reaction (PCR) polyacrylamide gel electrophoresis silver nitrate (AgNO3) staining were used to detect DNA fragments of both 159～196 bp and 336 bp under conditions of different concentration and temperatnre developed solution.Results:The DNA fragments detected by the modified method of silver staining were clear and suitable to analyze.Conclusion: Using silver staining to analyze the polyacrylamide gel bands contributes to a longterm preservation and can reduce the pollution effects on human beings and environment.更多还原