【摘要】 目的 :研究人胃癌BGC 82 3细胞系的遗传不稳定性。方法 :应用染色体G 分带、核型分析和PCR及银染技术。结果 :BGC 82 3细胞系染色体众数为亚三倍体。规律性出现 3个标记染色体 ,1号染色体短臂部分缺失。部分核型出现双微小体。微卫星位点D9s171为纯合性缺失 ,D9s16 0 4为杂合性缺失。结论 :BGC 82 3细胞系在染色体水平和DNA水平均表现遗传不稳定性更多还原

【Abstract】 Aim:To study the genetic instability of BGC 823 gastric cancer cell line. Methods:The techniques including G banding, karyological analysis, PCR amplification and silver staining were used.Results:The chromosome modal number exhibited as subtriploid. The 3 marker chromosomes and deletion of distal end of chromosome 1’s short arms (1p) were found with a very high incidence. The double minutes appeared in some mitotic cells. The microsatellite loci, D9s171 and D9s1604, demonstrated homozygous and heterozygous deletion,respectively.Conclusion: BGC 823 cell line exhibites genetic instability in chromosomal and DNA level.更多还原