【摘要】 目的 :以逆转录聚合酶链反应 (RT PCR)检测外周血大肠癌细胞 ,探讨大肠癌患者外周血中癌细胞存在的临床意义。方法 :应用RT PCR方法检测 47例不同Dukes分期结肠癌患者及其肝转移患者外周血中大肠癌细胞角蛋白 2 0 (CK2 0 ) ,并以大肠癌组织 (2 0例 )和正常健康人外周血 (2 0例 )为对照。结果 :正常健康人外周血中未见CK2 0mRNA表达 ,大肠癌患者外周血中CK2 0mRNA检出率达 40 4% (19/ 47) ,DukesB ,C ,D期患者外周血CK2 0mR NA检出率分别为 2 5 0 % (5 / 2 0 ) ,41 2 % (7/ 17)和 87 5 % (7/ 8) ,(P <0 0 5 )。肝转移患者CK2 0mRNA检出率为87 5 % (7/ 8) ,无肝转移患者检出率为 30 8% (12 / 39) (P <0 0 1)。结论 :CK2 0可作为肿瘤标志物 ,用于检测外周血中的大肠癌细胞 ,预测肿瘤复发及转移 ,识别高危患者更多还原

【Abstract】 Aim: To assess the clinical significance of the colorectal cancer cells in peripheral blood with reverse transcription polymerase chain reaction(RT-PCR)Methods:47 patients with colorectal cancer in different Dukes stages were examined the expression of cytokeretin 20(CK20) in cancer cells in the peripheral blood chosen by RT-PCR,which was compared with that of the tissue of colorectal cancer (20 cases).Results:In the normal person,the CK20 mRNA expression in peripheral blood had not been revealed,but the positivity rate of CK20 mRNA (in peripheral blood of patients with colorectal cancer) was 40.4%(19/47),and those in patients with Dukes stage B,C,D were 25.0%(5/20),412%(7/17) and 875%(7/8),respectively (P<005)The positivity rate of CK20 mRNA in patients with hepatic metastasis was 87.5%(7/8),while 30.8%(12/39)in patients without hepatic metastasis (P<001)Conclusion:CK20 may be a tumor marker for detecting colorectal cancer cell in peripheral blood as well as tumor relapse and metastasis.更多还原