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大肠癌患者外周血中癌细胞角蛋白20的检测

Detection of cytokeretin 20 in peripheral blood of patients with colorectal carcinoma

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【作者】 刘新郑师玉海李遂柱

【Author】 LIU Xinzheng 1),SHI Yuhai 2),LI Suizhu 3) 1)Department of Clinical Laboratory,the First Affiliated Hospital,Henan Medical University,Zhengzhou 450052 2)Department of Surgery,the People’s Hospital of Huaiyang,Huaiyang 466700 3)Genetic Center,the Thi

【机构】 河南医科大学第一附属医院检验科!郑州450052淮阳县人民医院外科!淮阳466700河南医科大学第三附属医院遗传中心!郑州450052

【摘要】 目的 :以逆转录聚合酶链反应 (RT PCR)检测外周血大肠癌细胞 ,探讨大肠癌患者外周血中癌细胞存在的临床意义。方法 :应用RT PCR方法检测 47例不同Dukes分期结肠癌患者及其肝转移患者外周血中大肠癌细胞角蛋白 2 0 (CK2 0 ) ,并以大肠癌组织 (2 0例 )和正常健康人外周血 (2 0例 )为对照。结果 :正常健康人外周血中未见CK2 0mRNA表达 ,大肠癌患者外周血中CK2 0mRNA检出率达 40 4% (19/ 47) ,DukesB ,C ,D期患者外周血CK2 0mR NA检出率分别为 2 5 0 % (5 / 2 0 ) ,41 2 % (7/ 17)和 87 5 % (7/ 8) ,(P <0 0 5 )。肝转移患者CK2 0mRNA检出率为87 5 % (7/ 8) ,无肝转移患者检出率为 30 8% (12 / 39) (P <0 0 1)。结论 :CK2 0可作为肿瘤标志物 ,用于检测外周血中的大肠癌细胞 ,预测肿瘤复发及转移 ,识别高危患者

【Abstract】 Aim: To assess the clinical significance of the colorectal cancer cells in peripheral blood with reverse transcription polymerase chain reaction(RT-PCR)Methods:47 patients with colorectal cancer in different Dukes stages were examined the expression of cytokeretin 20(CK20) in cancer cells in the peripheral blood chosen by RT-PCR,which was compared with that of the tissue of colorectal cancer (20 cases).Results:In the normal person,the CK20 mRNA expression in peripheral blood had not been revealed,but the positivity rate of CK20 mRNA (in peripheral blood of patients with colorectal cancer) was 40.4%(19/47),and those in patients with Dukes stage B,C,D were 25.0%(5/20),412%(7/17) and 875%(7/8),respectively (P<005)The positivity rate of CK20 mRNA in patients with hepatic metastasis was 87.5%(7/8),while 30.8%(12/39)in patients without hepatic metastasis (P<001)Conclusion:CK20 may be a tumor marker for detecting colorectal cancer cell in peripheral blood as well as tumor relapse and metastasis.

  • 【分类号】R735.34
  • 【被引频次】1
  • 【下载频次】20
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