【摘要】 本文利用核质互作与辐射处理研究了促进优质HMW GS基因的转化。结果表明 ,不同组合处理间F1籽粒优质亚基 (Glu D15+1 0 )的转化存在差异 ,辐射处理的多以杂合型出现。F2 分离群体中 ,反交及反交结合辐射处理的抽穗期明显倾向于早熟的亲本 ,细胞质与辐射具有促进抽穗期的作用。F3 植株Glu D15+1 0亚基的表达频率 ,不论是哪种组合 ,反交时 5+1 0亚基的表达频率高于正交 ;无论正交还是反交 ,辐射处理提高了 5+1 0亚基的表达频率 ,而且在不同组合间存在差异。更多还原

【Abstract】 Irradiation treatment and the interaction of nuclear and cytoplasm were employed to promote the transformation of HMW\|GS gene with high wheat quality. The results showed that there were differences among treatments in transformation of high quality gene(Glu\|D 1 5+10) in F 1 grains, and heterozygous type was more frequently appeared in the treatment of irradiation. In the F 2 segregative population, heading date deviated to early\|mature parent in the treatment of reciperocal cross and its combination with irradiation, indicating that cytoplasm and irradiation could promote the heading. In F 3 the expressive frequency of (5+10) gene in the cross was lower than that in reciperocal cross, and irradiation treatment could raise the expressive frequency of(5+10) gene. These results showed varience in different crosses.更多还原