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反相高效液相色谱法分离和测定亚麻植株中植物激素
Separation and Determination of Plants Hormones in FLax Plant With Reversed-phase High Efficient Liquid Chromatography
【摘要】 探讨了用反相高效液相色谱法分离和测定经不同除草剂处理的亚麻植株中两种生长激素吲哚已酸 (IAA)与赤霉素 (GA3)的方法。以甲醇、乙腈和水为流动相 ,5分钟可将两种激素快速分离和定量测定 ,线性关系良好 ,回收率较高
【Abstract】 In this paper, reversed-phase high efficient liquid chromatography was used for the separation and determination of plant hormones indole-3-acetic acid(IAA) and gibberellic acid(GA 3) in flax plantstreated with different herbicides.Methanol, acetonitril and water system was chosen as the mobile phase. Within 5 minutes two plant hormones can be separated and determinated. The caliration curves for the two compounds had a lineal relation. The rete of recycle was very high.
【关键词】 亚麻植株;
吲哚乙酸(IAA);
赤霉素(GA3);
液相色谱法;
分离和测定;
【Key words】 flaxp lants; indole-3-acetic acid(IAA); gibberellic acid(GA3); liquid chromatography; separation and determinatied;
【Key words】 flaxp lants; indole-3-acetic acid(IAA); gibberellic acid(GA3); liquid chromatography; separation and determinatied;
- 【文献出处】 黑龙江农业科学 ,Heilongjiang Agricultural Science , 编辑部邮箱 ,2001年06期
- 【分类号】Q946.885;Q-331
- 【被引频次】34
- 【下载频次】362