【摘要】 以RT -PCR方法扩增出TGEV纤突糖蛋白 (S)N端基因片段 ,它包含D、C两个抗原位点 ,长 1 2kb ,平端连接方法将其与质粒PUC18的ECORⅠ多克隆位点相连 ,电转化DH5α ,经筛选鉴定获得阳性基因克隆更多还原

【Abstract】 Transmissible gastroenteritis is an economically important disease in China swine herds The disease produces illness and death losses in young piglet and export markets are closed to breeding stock from TGEV positive herds Molecular techniques were developed to rapidly identify the TGEV The techniques developed are important aids to diagnostic laboratoried and university researchers as a first step in generating a control plan for TGEV disease outbreaks and tracking the spread of the virus N terminal of S gene was cloned into plasmid PUC18 in E coli DH5 α by blunt ligation更多还原