【摘要】 建立了一种新的测定Fenton反应所产生的羟自由基的方法 ,苯基荧光酮 (Phenylfluorone ,简称本芴酮 )与Fenton试剂作用后 ,使其荧光大大降低 ,其最大激发波长和发射波长分别为 50 0nm和 42 0nm ,基于苯基荧光酮在反应前后的荧光变化 ,即可间接地测定羟自由基的产生量。同时 ,通过清除率实验反证了该方法的可靠性 ,利用顺磁共振法测定苯基荧光酮加入前后的波谱变化来进一步证实该方法的正确性。化学测定过程均在国产仪器下完成 ,具有较高的灵敏度。可推广为一种作为寻找羟自由基清除剂的方法 ,也可作为医学上部分药品性能检验的一种方法更多还原

【Abstract】 This article developed a new method for determination of the hydroxyl radicals in Fenton reaction.After Phenylfluorone react with Fenton reagent,the Fluorescence is increased and excitation and emission wavelength are at 500nm and 420nm.With the change of fluorescence intensity ,it can be used to determine by studying the determination conditions.At the same time,through the improvement of clearing experiment and ESR, the new method is stable and correct.The chemical analysis can be finished by using indigenous instruments.The method has a high sensibility and can be used as a method to look for removal reagent and detect the performance of some medicine.更多还原