【摘要】 目的通过MGIT960培养系统结合国外最新的INNO -LIPATM RIF.TB(LIPA)DNA探针技术的应用 ,探讨其快速诊断结核病的可行性。方法应用BACTECMGIT960系统进行临床疑似结核病例标本的培养 ,筛选了70例结核分枝杆菌 ,35例非结核分枝杆菌 ,用LIPA探针技术诊断是否为结核分枝杆菌。结果用LIPADNA方法在70例结核分枝杆菌中获得阳性69例 ,占98.6 % ;35例非结核分枝杆菌均为阴性。与单纯消化纯化后涂片的82.9 % (58/70)的阳性率相比增加了15.7个百分点 ,两者在统计学上差异有显著性(P<0.05)。BactecMGIT960培养结合LIPADNA探针与传统诊断方法4～8周相比 ,整个过程只需6～37天 ,平均15天 (2周 )。结论LIPADNA探针是一种具有高度敏感性和特异性、能够简便、快速、准确地诊断结核病的新技术。同时还能鉴定rpoβ 基因位点的突变 ,诊断耐利福平 (RMP)性结核 ,有望适用于结核分枝杆菌耐RMP性结核的快速检测 ,进一步为结核病快速准确的治疗提供理论依据。更多还原

【Abstract】 Objective To research the feasibility of rapid and accurate diagnosing Mycobacterium tuberculosis through the application of new technology Bactec MGIT culture system combining with the INNO_LIPATM RIF.TB (LIPA) DNA probe. Methods Applied Bactec MGIT 960 system inoculated the clinical specimens and selected the 70 Mycobacterium tuberculosis (MTB) and 35 Non_Tuberculosis Mycobacterium (NTM).Then LIPA probe was used to identify whether MTB.Results The positive rate for LIPA probe was 98.6% (69/70) and the smear was 82.9% (58/70).Statistical analysis found significant difference between the smear and LIPA DNA probe.Bactec MGIT 960 system combined with the LIPA probe only needed the 6～37 turnround days and mean time was 15 days for diagnosis of MTB.Conclusion Conjunction with the MGIT 960 system and LIPA PCR_DNA probe is the new technology of high sensitivity and spe_cificity enough, which can rapid and accurate diagnoses Mycobacterium tuberculosis and identify the rpoβ gene mutation at same time, can also provide theory for the rapid and accurate treatment of rifimpicin resistance for Mycobacterium tuberculosis. [更多还原