【摘要】 目的 :探讨尿 5 ’-核苷酸磷酸二酯酶 (5 ’- NPD)能否反映顺铂导致的早期肾损害。方法 :采用完全随机设计方法 ,建立顺铂致大鼠肾损害动物模型 ,通过检测顺铂致大鼠肾损害过程中尿 5 ’- NPD的活性变化 ,同时用光镜和电镜观察大鼠肾脏的病理改变 ,以验证尿 5 ’- NPD能否反映早期肾损害。结果 :实验中空白对照组 (IV组 )和小剂量顺铂组 ( 组 )大鼠的尿5 ’- NPD活性以及肾脏病理组织学均无明显改变 ;大剂量顺铂组大鼠 ( 组和 组 )在投药后第 2天 ,尿 5 ’- NPD活性开始升高 ,第 4天到达高峰 ,活性明显高于 组和 组 ,而肾脏的病理改变则出现得相对迟缓 ,投药后第 8天可见肾小管中度扩张 ,部分区域有炎性细胞浸润 ;第 15天有较多肾实质细胞坏死。结论 :尿 5 ’- NPD指标对顺铂导致的肾损害反映较灵敏 ,可望作为监测顺铂致早期肾损害的指标更多还原

【Abstract】 Objective:To investigate whether the activity of 5’ -nucleotide phosphodiesterase (5’-NPD) in urine of rats could be used as a marker for monitoring the early dam age of kidney induce by cisplatin (DDP) Methods:T he rats model of kidney-damage induce by DDP was established After the rats were treated with DDP, the change s of 5’-NPD activity in urine samples were measured and the histopathologic chan ges of kidney were observed under light and electric microscopes to decide firmly the relationship between 5’-NPD activity and the early damage of kidney induce by DDP Results:The rats were randomly divided into high dose DD P group,low dose DDP and control group The activity of 5’-NPD in urine and the histopathol ogic changes of the kidney were not significantly different between low dose DDP group and control group in the experiment The activity of 5’-NPD in urine incr eased on the second day after high dose DDP administration and the highest level was found on the 4th day It’s higher than that of the group of control and low dose DDP The histopathologic changes of kidney occurred later Moderate dilati on in renal tubules and inflammatory cell infiltration was found in some areas of rat’s kidney on the 8th day The necrosis occurred in many renal parenchyma cellson the 15th day Conclusion:The level of 5’-NPD in urine sample can reflect sensitively the damage of kidney induced by DDP and can be u sed as a maker for monitoring the early damage of kidney induced by DDP更多还原