【摘要】 DNA芯片技术是近年迅速发展的一门生物高新技术 ,其突出特点在于高度并行性、多样性 ,即能一次性对生物遗传信息进行大规模的快速、同步分析。DNA芯片技术目前已用于基因重复测序、基因表达分析、新基因的发现、基因单核苷酸多态性 ( SNPs)研究、基因诊断、药物筛选等领域 ,而且其应用范围还在不断扩展。本室运用这一新技术 ,检测水稻在受水稻黄单胞菌水稻致病变种及该菌株的 rpf C基因缺失突变体感染时基因表达的差异 ,发现一些基因可能与水稻抗白叶枯病相关更多还原

【Abstract】 DNA microarrays or chips, a hot new biotechnique developed rapidly in recent years, enables biologists to monitor gene expression on largescale and gain a great deal of genetic information at the same time. By this novel approach, not only DNA sequencing and gene expression analysis but also gene diagnoses, study of gene singlenucleotide polymorphisms(SNPs) and new medicine screening can be performed.Forthermore,its use keeps expanding to many fields.With the technique of rice cDNA microarrays,we detected gene differential expression of rice infected with a wild type Xanthomonas oryzae pv.oryzae(Xoo for short) and a weakvirulent type Xoo rpfCdeletion mutant,respectively,and found some genes involved in resistance to the disease of the rice bacterial blight.更多还原