【摘要】 本研究建立了反转录聚合酶链反应 ( RT PCR)技术检测猪瘟病毒 ( Hog Cholera Virus,HCV)方法。合成的二对引物 HCV 1 / HCV 2和 HCV A1 / HCV A2成功地扩增兔化弱毒株、法国株和石门株的核酸 ,并对采自博白县 3个、贵港市 1个和武宣县 1个猪场共 1 3份病料进行检测。结果从博白县和贵港市的 1 1份材料中检测到 HCV的核酸。更多还原

【Abstract】 In this study reverse transcription coupled with the polymerase chain reaction(RT PCR) has been established to detect hog cholera virus (HCV). Two pairs of primers,HCV1/HCV2 and HCVA1/HCVA2,has been synthesized and successfully to amplify nucleic acids of HCLV strain, France strain and Shimen strain of HCV. In experiment proper,13 clinical samples were examined from three different locolities in Guangxi namely, Bobai, Guigang and Wuxuan constituting three, one and one pig farms respectively. As a result, 11 of 13 samples from Bobai and Guigang by both primers indicating that the sick pigs in which the sample was derived were infected by HCV.更多还原