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乙酸对DNA空间结构微观损伤的Raman光谱特征

Raman Spectroscopic Study of Microscopic Damage on the Space Structure of DNA with Acetic Acid

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【作者】 柯惟中余多慰顾柏平庄振武

【Author】 KE Wei zhong 1,YU Duo wei 2,GU Bai ping 3,and ZHUANG Zhen wu 4 1 Physics and Chemistry Centre,Jiangsu Provincial Key Lab for Optoelectric Technology,Nanjing Normal University,Nanjing 210097,China; 2 Department of Biology,Jiangsu Provincial Ke

【机构】 南京师范大学理化中心南京师范大学生物系江苏省资源生物技术重点实验室南京中医药大学江苏省人民医院 江苏省光电技术重点实验室江苏南京2100979江苏南京210097江苏南京210029江苏南京210029

【摘要】 利用Raman光谱技术从分子水平研究了乙酸对鲱鱼精DNA空间结构微观损伤的特征。实验结果表明 ,鲱鱼精DNA被乙酸浸润处理后 ,Raman特征频率与强度均发生了不同程度的变化。证明了乙酸对DNA的损伤是整个分子 ,包括骨架磷酸基团、脱氧核糖及碱基。不仅是DNA的构象 ,而且其构型也发生了变化 :氢键断裂 ,B型构象被修改 ,DNA单、双链断裂 ,碱基也受到了严重损伤。其碱基损伤严重程度依次为胞嘧啶、胸腺嘧啶、鸟嘌呤和腺嘌呤。

【Abstract】 The character of microscopic damage on the space structure of the herring sperm DNA by wetting treatment of acetic acid with Raman spectroscopic technique was reported.The Raman characteristic frequency and intensity were changed in different degree after wetting treatment on DNA by acetic acid.The results showed that the entire DNA molecule was damaged by acetic acid including backbone phosphodiester groups,deoxyribose and base stacking.The conformation and forms of DNA were changed,hydrogen bond was broken,B form was modified,both single and double chains were disrupted.Base pairs were damaged.The damage degrees of the base pairs in turn were cytosine,thymine,guanine and adenine.

【关键词】 拉曼光谱鲱鱼精DNA乙酸
【Key words】 Raman spectraHerring sperm DNAAcetic acid
【基金】 江苏省科委自然科学基金;江苏省高校重点实验室开放基金资助
  • 【文献出处】 光谱学与光谱分析 ,Spectroscopy and Spectral Analysis , 编辑部邮箱 ,2001年06期
  • 【分类号】O657.37
  • 【被引频次】13
  • 【下载频次】159
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