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荧光光谱法研究低频脉冲电场对胰岛素分子的作用
Effects of Low Frequency Pulsed Electric Field on Insulin Studied by Fluorescent Spectrum
【摘要】 胰岛素作为一种胞间信号分子 ,对于生命信息的传递起着极为重要的作用。探讨低频脉冲电场对胰岛素分子的作用 ,对于从细胞信号系统的角度来解释生物电磁效应有着十分重要的意义。在实验中 ,对照组和场暴露组之间荧光峰值强度的差别表明了低频脉冲电场改变了胰岛素分子的构象 ,低频脉冲电场可能通过破坏胰岛素分子内的部分氢键和重新形成某些氢键来改变它的构象。本文结果表明胞间信号分子也是低频电磁场作用的一个重要靶体
【Abstract】 Insulin,an intercellular signal molecular,plays a critical role in trasferring life information.The studies on effects of pulsed electric fields on insulin molecular are meaningful for explaining the mechanism of biological effects of electromagnetic fields.The experiment results demonstrate that the conformation of insulin molecular has been altered by electric fields exposure for 30 minutes or by heat exposure for 50 hours.The results suggest that pulsed electric field exposure as heat exposure will alter conformation of insulin molecular by breaking some hydrogen bonds and recombining another hydrogen bonds.The intercellular signal molecular may be an important target of electromagnetic fields.
【Key words】 Fluorescent peak intensity; Insulin; Low frequency pulsed electric fields; Conformation;
- 【文献出处】 光谱学与光谱分析 ,Spectroscopy and Spectral Analysis , 编辑部邮箱 ,2001年03期
- 【分类号】O657.3
- 【被引频次】11
- 【下载频次】89