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柿品种‘禅寺丸’未减数花粉分离体系的建立

Study on the Establishment of Separation System for Unreduced Pollens in ‘Zenjimaru’ Persimmon Variety

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【作者】 谷晓峰唐仙英罗正荣

【Author】 Gu Xiaofeng,Tang Xianying,and Luo Zhengrong Department of HorticultureHuazhong Agricultural UniversityWuhan,Hubei 430070

【机构】 华中农业大学园艺系!湖北武汉430070

【摘要】 对柿品种’禅寺丸’未减数花粉(2n花粉)的鉴定、筛选及其生活力恢复进行了研究,结果表明:(1)经0.9 mol/L甘露醇CPW溶液吸胀2h后,正常花粉直径为50 μm左右,而未减数花粉为65 μm左右,未减数花粉直径是正常花粉的1.3倍。(2)未减数花粉比率约为3.8%,通过61μm的不锈钢细胞筛筛选,纯度可达84%。(3)禅寺丸花粉经吸胀处理后,花粉发芽率有所下降,利用20%的蔗糖溶液洗涤可基本恢复其生活力。

【Abstract】 Studies were carried out on the identification,screening and recovery of viability of unreduced pollen (2n pollen) in Japanese ’Zenjimaru’ persimmon variety  Diospyros kaki L. f .The results showed that 1 The diameter of normal pollen and unreduced pollen was 50 μm and 65 μm,respectivelyafter imbibed about 2 hours in CPW solution added 0.9 mol/L mannitol. Unreduced pollen was 1.3 times to normal one in diameter.2Unreduced pollen percentage was 3.8% naturally and could be increased to 84% after screened by stainless steel mesh.3The viability of pollen declined after imbibed  but could recover partly by 20% sucrose solution.

【基金】 国家自然科学基金;高等学校博士学科专项科研基金
  • 【文献出处】 果树学报 ,Journal of Fruit Science , 编辑部邮箱 ,2001年01期
  • 【分类号】S665.2
  • 【被引频次】29
  • 【下载频次】197
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