【摘要】 本文探讨了钙、镁、铜等 7种微量元素对维生素 B1、B2 、B6的同步荧光光谱的影响。实验结果表明 ,当Ca2 + 的浓度为 1.0× 10 -3mol/ L时 ,VB2 、VB6的同步荧光光谱有显著的增强作用 ;而其他微量元素对VB1、VB2 、VB6的同步荧光有不同程度的猝灭或增强作用。利用 Ca2 + 对 3种维生素荧光强度的增敏作用 ,可以有效地提高同步荧光分析法同时测定 VB1、VB2 、VB6的灵敏度 ,VB1、VB2 、VB6的校准曲线的线性范围分别为 2 .4× 10 -7— 9.0× 10 -6、1.1× 10 -7— 4 .6× 10 -6、7.1× 10 -7— 2 .0× 10 -6mol/ L。采用本方法测定混合样品 ,样品不需分离 ,操作简便、快速更多还原

【Abstract】 The effect of trace elements of Ca 2+ ,Mg 2+ and Cu 2+ on the synchronous fluorescence spectra of vitamin B 1,B 2 and B 6 were studied in this paper.When the concentration of Ca 2+ is 1.0×10 -3 mol/L,it can improve the fluorescence spectra of vitamin B 2 and B 6,while the other trace elements show quenching or enhancing effect on the synchronous fluorescence spectra of vitamin B 1,B 2 and B 6 in varying degrees.By the sensitivity enhancing effect of Ca 2+ ,the sensitivity of synchronous fluorescence measuring VB 1,VB 2 and VB 6 can be improved and the linear range of their correction curves is in the range of 2.4×10 -7 -9.0×10 -6 mol/L,1.1×10 -7 -4.6×10 -6 mol/L,7.1×10 -7 -2.0×10 -6 mol/L ,respectively.The proposed method was applied to determination vitamin B 1,B 2 and B 6 in complex vitamin B tablets with satisfactory result.更多还原