【摘要】 目的 肝细胞生长因子(hepatocyte growth factor receptor,HGF)是具有多种生物活性的细胞因子,通遇与受体结合转导生物学效应,发挥促细胞增生、促运动和促形态发生等作用。本实验目的在于观察增生性糖尿病视网膜病变(proliferative diabetic retinopathy,PDR)不同时期视网膜前膜(epiretinal membranes,ERM)中HGF受体的表达情况。方法 13例PDR患者行玻璃体切除术剥离视网膜前膜。采用免疫组化染色方法观察HGFR在ERM中的表达情况。结果 在7例Ⅳ、Ⅴ期ERM标本中,5例HGF受体阳性表达,阳性表达率为71.4％;6例PDRⅥ期增殖前膜标本中,有5例HGF受体阳性表达,阳性表达率为83.3％。HGF受体阳性表达率与PDR分期无显著性差别(P>0.05)。结论 HGF受体在PDR视网膜前增生膜中表达,提示HGF可能参与了PDR增生膜的形成。更多还原

【Abstract】 Objective To investigate the expression of HGFR in epiretinal membranes (ERM) of eyes with proliferative diabetic retinopathy (PDR) hepatocyte growth factor (HGF) is a multipotential cytokine, which can produce a range of responses in target cells. HGF exerts its actions through founction of the HGF receptor (HGFR), transmitting its multifunctional signals such as regulation of cell proliferation, motty, and morphogenesis. Methods Thirteen human epiretinal membranes were obtained from eyes undergone vitrectomy for PDR and observed by immunohistochemical examination to study the expression of HGFR. Results In 7 membranes of PDR-grade Ⅳ, Ⅴ, HGFR were expressed in 5/7 (71.4%); and 5 cases were detected in 6 membranes of PDR-grade Ⅵ (83.3%). No significant differences were found between the two groups in the expression of HGFR (P>0.05). Conclusion The findings indicated that HGF might be involved in the formation of epiretinal membranes in PDR.更多还原