【摘要】 采用反向嵌套PCR法 ,根据已获得的辽宁碱蓬甜菜碱醛脱氢酶cDNA的部分序列 ,设计一条 5′末端磷酸化的特异性反转录引物和两对特异性反向嵌套PCR引物 ,成功地克隆了辽宁碱蓬甜菜碱醛脱氢酶cDNA 5′末端。与锚定PCR法相比 ,反向嵌套PCR法具有特异性强、扩增效率高等优点 ,是一种非常有效的扩增cDNA 5′末端序列的方法。更多还原

【Abstract】 Inverse nested PCR can rapidly amplify 5′cDNA ends by using a specific RT primer whose 5′end is phosphated and two pairs specific Inverse nested PCR primers. In this reseach,the 5′cDNA ends of S.Liaotungensis betaine aldehyde dehydrogenase is cloned using this method.Contract with the Anchored PCR,Inverse nested PCR has better specificity and higher amplificaton effects.It is a very effective method of amplifying 5′cDNA ends.更多还原