【摘要】 目的 :体外定向诱导兔骨髓间质干细胞 (MSC)分化为神经元样细胞。方法 :采用Ficoll Paque液 (1 .0 83g/L)离心分离兔MSC ,体外扩增 ,硫代甘油等试剂的无血清达乐伯克改良必需基本培养基 (DMEM)诱导MSC分化为神经元。免疫组化鉴定神经元烯醇化酶 (NSE)、神经丝蛋白 (NF)、胶质纤维酸性蛋白 (GFAP)的表达。结果 :兔骨髓间质干细胞在体外扩增原代可获得 5× 1 0 5、1 0代可获得 2× 1 0 1 0 个细胞。加入硫代甘油诱导后 ,MSC胞体收缩 ,突起伸出 ;免疫组化显示诱导出的神经元样细胞NSE、NF、表达阳性 ,GFAP阴性。结论 :兔骨髓间质干细胞在体外可以分化为神经元样细胞更多还原

【Abstract】 Objective:To induce the differentiation of rabbit bone marrow mesenchymal stem cells (rMSC) into neuron like cells in vitro. Methods: rMSCs were separated from rib marrow with Ficoll Paque reagent and expanded in culture medium. Differentiation of rMSCs into neurons were induced with DMEM/monothioglycerol. Neuron specific enolase(NSE),neurofilament (NF),glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. Results:rMSCs were expanded in culture for 10 passages.After the induction by adding monothioglycerol the MSCs displayed a fibroblast like morphology. Immunohistochemical assay showed that there were positive expressions of NSE and NF M,on the neuron like cells,without the expression of the glial astrocyte marker GFAP.Conclusion:It suggests that the differentiation of rMSC into neurons can be induced in vitro.更多还原