【摘要】 以广东阳江鲨鱼软骨为原料 ,研究了鲨鱼软骨新生血管形成抑制因子的分离纯化方法 ,并对其生物学活性进行了初步研究 .采用盐酸胍抽提、膜超滤、丙酮分级沉淀、Sephadex G-75柱层析和 C4反相高效液相色谱等步骤 ,分离纯化出一种新的鲨鱼软骨血管生成抑制因子 -a( Shark Cartilage Angiogenesis Inhibitor-a,SCAI-a) ,分子量为 1 2 60 0 ,能显著抑制鸡胚绒毛尿囊膜血管的形成更多还原

【Abstract】 The purification method and bioactivity of shark cartilage angiogenesis inhibitor a(SCAI a) from Guangdong Yangjiang were investigated. The 1.0 mol/L guanidine and 0 02 mol/L MES(at 28 ℃ ) extracts of shark cartilage was centrifuged at 6 000 r/min for 30 min and the pellet was discarded, and the supernatant was recentrifuged at 12 000 r/min for 30 min, then ultrafiltrated and fractionally precipitated with 25%-90% acetone. The precipitate was applied to a Sephadex G 75 gel column and eluted after the predominant peak of A 280 nm absorbing material with 0.05 mol/L Tris HCl buffer(containing 0.02 mol/L NaCl and 0.02% NaN 3). The material B were composed of three single peptides with molecular mass of 18 000, 15 000 and 12 600, then were purified using reverse phase high performance liquid chromatography, the eluate was collected, dried and observed by chichen chorioallantoic membrane. The pure inhibitor was homogeneous as a single band on a silver stained 12 5% sodium dodecyl sulfate polyacrylamide gel. SCAI a had a molecular weight of 12 600. It specifically inhibited the chorioallantoic membrane of chick embryos.更多还原