【摘要】 用胶束电动力学毛细管色谱法测定尿中核苷 .通过苯基硼酸亲和色谱柱对尿中核苷进行纯化 ,以十二烷基磺酸钠 ( SDS) -十水合四硼酸钠 -磷酸二氢钠为缓冲液 ( p H=6.96) ,在未涂层石英毛细管上 ,于 7k V恒压 ,2 9℃下进行电泳分离 ,检测波长为 2 54nm.此方法用于 2 8个正常人尿中 1 4种核苷的测定 ,确定了正常成年人 (汉族 )尿中核苷的排放范围 .更多还原

【Abstract】 A micellar electrokinetic chromatography method for identification and quantitation of nucleosides in urine has been optimized. After nucleosides were extracted from human urine on phenyl boronic acid affinity chromatography column, the analysis was performed in an uncoated 52 cm×50 μm i.d. capillary tube using a 300 mmol/L SDS 25 mmol/L borate 50 mmol/L phosphate buffer (pH=6 96), 0 5 Psi×15 s injection, 7.0 kV voltage and UV detection at 254 nm. By using the MEKC method developed, 14 normal and modified nucleosides in 28 normal urines were quantified to establish the reference ranges. The result shows that this MEKC method is reliable, sensitive, efficient and suitable for clinical application.更多还原