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金属离子导致的丝素蛋白的构象转变

The Effect of Metal Ions on the Conformation Transition of Silk Fibroin

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【作者】 李贵阳周平孙尧俊姚文华宓泳姚惠英邵正中于同隐

【Author】 LI Gui Yang 1, ZHOU Ping 1* , SUN Yao Jun 2, YAO Wen Hua 2, MI Yong 3 YAO Hui Ying 3, SHAO Zheng Zhong 1, YU Tong Yin 1 (1. Key Laboratory of Molecular Engineering of Polymers, Ministry of Education, 2. Center for Analysis and

【机构】 复旦大学聚合物分子工程教育部重点实验室复旦大学分析测试中心复旦大学应用离子束教育部重点实验室!上海200433复旦

【Abstract】 How the silkworm spins out such an excellent silk fiber has been a hot topic. The issue here is to know how the coil chains in the gland of silkworm transform to β sheet in silk fiber. We used the regenerated silk fibroin to imitate the silk fibroin of the gland to investigate the effect of metal ion(Ca 2+ . Cu 2+ ) on the silk spinning process of Bombyx mori silkworm. The solutions or gel obtained from hte mixture of regenerated silk fibroin and metal ions were dried for mimicking the gradual loss of water in the spinning process. The 13 C NMR spectrum simulation for C β nucleus of alanine quantitatively demonstrated that the conformation of the regenerated silk fibroin is dominantly of silk Ⅰ, whereas the fibroin with metal ions has more silk Ⅱ conformation. Raman spectroscopies show the consistent results with that of NMR. The binding of metal ion with carbonyl and amide in protein backbone allows the protein chain to array regularly, therefore leading to the β sheet formation.

【关键词】 丝素蛋白构象转变NMR金属离子
【Key words】 Silk fibroinConformation transitionNMRMetal ions
【基金】 国家自然科学基金! (批准号 :2 99740 0 4);教育部青年骨干基金;上海市自然科学基金! (批准号 :99ZA14 0 0 1;波谱与原子分子物
  • 【文献出处】 高等学校化学学报 ,Chemical Research In Chinese Universities , 编辑部邮箱 ,2001年05期
  • 【分类号】O629
  • 【被引频次】72
  • 【下载频次】444
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