【摘要】 将半导体量子点应用于标记天花粉蛋白 ,研究了量子点标记天花粉蛋白的吸收光谱、荧光光谱和酶活性变化 .与游离的 QDs相比 ,QDs-TCS的吸收光谱在 4 0 0～ 60 0 nm范围内不发生明显变化 ;QDs-TCS的发射光谱发生蓝移 ,但发射半宽不变 ;标记上 QDs后 TCS的酶活性没有发生改变 .利用双光子激发扫描荧光显微镜和激光共聚焦显微镜同时观察 QDs-TCS在人绒癌细胞内的分布发现 ,QDs-TCS能够跨膜进入细胞 ,并在细胞核周围呈聚集分布 .更多还原

【Abstract】 Semiconductor quantum dots were used for the first time to label trichosanthin. The absorption spectrum, fluorescent spectrum and enzymatic activity of quantum dots-labeled trichosanthin were studied in this paper. The absorbane of QDs-TCS varied to a less extent with wavelength in the range of 400\₆00 nm. The fluorescent spectrum of QDs-TCS underwent a blue-shift in the emission peak, but the intrinsic spectral width was unchanged. No changes were found in the enzymatic activity of QDs-TCS in comparison with trichosanthin. Distribution of QDs-TCS within human choriocarcinoma cells was simultaneously observed with two-photon laser scanning microscopy and confocal laser scanning microscopy. The results indicated that QDs-TCS could enter the cytoplasm across the membrane and aggregated around the nuleolus.更多还原