【摘要】 目的　动态观察卵巢肿瘤患者血清血管内皮生长因子 (VEGF)的水平变化及其临床意义。方法　应用双抗体夹心酶联免疫吸附法 (ELISA)分别检测正常健康妇女 (正常组 ,38例 ) ,卵巢良性肿瘤妇女 (良性组 ,42例 )和卵巢恶性肿瘤妇女 (恶性组 ,73例 )手术前后及卵巢恶性肿瘤妇女术后化疗后 (随访组 ,47例 )外周血清中VEGF的水平。结果　恶性组VEGF水平明显高于良性组和正常组 (P <0 .0 1) ,良性组与正常组之间差异无显著性 (P >0 .0 5 )。恶性组临床分期晚 (Ⅲ ,Ⅳ期 )的患者VEGF水平明显高于临床分期早的 (Ⅰ ,Ⅱ期 ,P <0 .0 1) ,但与肿瘤病理组织学类型、病理分级和患者年龄无明显关系 (P >0 .0 5 )。恶性组手术后VEGF水平明显低于手术前 ,良性组手术前后VEGF水平差异无显著性 (P >0 .0 5 )。随访组 35例未复发者动态检测VEGF 2 78次 ,其平均值为 ( 4 7.32± 2 1.71)ng/L ,随访期间复发或长期带瘤者 12例动态检测VEGF 6 4次 ,其平均值为 ( 4 39.5 8± 14 6 .17)ng/L ,与恶性组差异无显著性 (P >0 .0 5 ) ,但明显高于未复发者 (P <0 .0 5 )。结论　VEGF与卵巢癌的恶性行为密切相关 ,动态检测卵巢恶性肿瘤患者外周血VEGF的水平变化有可能作为卵巢癌疗效评价、复发转移及预后的重要指标更多还原

【Abstract】 Objective To investigate the changes and clinical significance of serum vascular endothelial growth factor (VEGF) concentrations in patients with ovarian carcinoma.Methods Serum VEGF concentrations in 38 healthy women (normal group), 42 patients with benign ovarian carcinoma (benign group), 73 patients with malignant ovarian carcinoma (malignant group) and 47 follow-up malignant ovarian carcinoma patients (follow-up group) were measured by sandwich enzyme-linked immunoadsorbent assay (ELISA).Results Serum levels of VEGF were significantly higher in malignant group than those in benign group and normal group ( P <0.01), but there was no significant difference between benign and normal group ( P >0.05). In malignant group, statistical analysis showed that serum VEGF levels in clinically advanced patients (stage Ⅲand Ⅳ) were significantly higher than those in early-stage (stageⅠand Ⅱ) patients ( P <0.01), but did not show significant difference in the levels of VEGF among ovarian carcinomas classified according to histopathologic types and grades. Serum VEGF levels decreased significantly ( P <0.05) in malignant group after optimal removal of tumor bulks. In benign group, there was no significant difference in the levels of VEGF before and after operation ( P >0.05). Of 47 follow-up patients, 35 patients did not have recurrence within follow-up period and 278 serum VEGF determination were carried out. The mean concentration was (47.32±21.71) ng/L in patients without relapse. On the other hand, otherwise, the mean serum VEGF level of 12 patients with recurrent or metastatic diseases was (439.58±146.17) ng/L, significantly higher than that of patients without recurrence.Conclusion These results suggest that VEGF may play an important role in the progression and metastasis of ovarian carcinoma, and serial determination of serum VEGF may be an important index for evaluation of efficiacy, recurrence, metastasis and prognosis for patients with malignant ovarian carcinoma.更多还原