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豚鼠过敏性休克类胰蛋白酶活力测定

The changes of tryptase activity in anaphylactic shock guinea-pigs

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【作者】 郭薇; 陈玉川; 刘水平; 成建定;

【Author】 GUO Wei, CHEN Yu-chuan, LIU Shui-ping,et al.(Department of Forensic pathology, Sun Yat-sen University of Medical Sciences, Guangzhou, 510080 )

【机构】 中山医科大学法医病理学教研室; 中山医科大学法医病理学教研室 广东广州510080; 广东广州510080; 广东广州510080;

【摘要】 目的探索过敏性休克法医学客观诊断标准。方法建立豚鼠异种血清过敏性休克模型,采用专性底物对模型鼠的血清,肺,气管类胰蛋白酶活力进行测定。结果过敏性休克豚鼠血清,肺,气管类胰蛋白酶活力均增加,且三者增加的程度是平行的。结论过敏性休克时,血清,肺,气管类胰蛋白酶活力增加可作为法医检案客观诊断标准。

【Abstract】 Objective To investigate the changes of the activity of tryptase of sera, lungs and bronchial tubes in the guinea-pigs which suffered from hetero-serum anaphylactic shock. Methods Sera and tissues were collected from anaphylactic shock guinea-pigs,and the enzyme activity was tested colormetrically using special substrate,BAPNA.Results The activity of tryptase of sera,lungs and bronchial tubes increased significantly in Anaphylactic guinea-pigs compared with control group.Conclusion The changes of tryptase activity are helpful to diagnose anaphylactic shock.

  • 【文献出处】 法医学杂志 ,Journal of Forersic Medicine , 编辑部邮箱 ,2001年04期
  • 【分类号】D919
  • 【被引频次】23
  • 【下载频次】357
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