【摘要】 目的 检测病毒性心肌炎（ viral myocarditis,VMC）猝死者心、脾组织柯萨奇 B₃病毒 （CVB₃）基因，探索 VMC的病原学诊断方法。方法 运用原位逆转录 PCR技术检测实验组（ VMC猝死， 8例）及对照组（非心性死亡， 4例）心、脾组织中的 CVB₃基因。结果 实验组中， 3例（第 1， 4， 7例）心肌 CVB₃基因阳性， 4例（第 2， 4， 6， 7例）脾组织 CVB₃基因阳性；对照组心、脾 CVB₃基因均为阴性。结论 心、脾组织中 CVB₃基因联合阳性可能是 VMC的重要特征；联合检测心、脾中 CVB₃基因可提高 VMC的病原学诊断率。更多还原

【Abstract】 Objective To detect the Coxsackie virus B₃（CVB₃） gene in myocardium and spleen tissues in viral myocarditis（VMC） with sudden death and to explore the diagnostic method for VMC by means of seeking pathogene.Methods By in situ RT－ PCR,the detection of CVB₃ gene in myocardium and spleen sections were performed in sudden death group caused by VMC and non－ cardiac death group.Results In VMC group,CVB₃ gene－ positive signals were seen in myocardium sections（3 out of total 8 cases， No.1,4,7 cases）and spleen sections（4 out of total 8 cases,No.2,4,6,7 cases）.In non－ cardiac death group,no positive signals were detected in both myocardium and spleen tissues.Conclusion Positive detection of CVB₃ gene in both myocardium and spleen maybe an important character of VMC and can improve the detecting pathogene in diagnosing VMC. [更多还原