【摘要】 以碳糊为固定化载体 ,将葡萄糖氧化酶固定在碳糊电极上 ,制成了光导纤维电化学发光葡萄糖生物传感器。葡萄糖的酶催化反应、鲁米诺的电化学氧化和化学发光反应在电极表面同时发生 ,因此该传感器的信号响应在 1 0 s内达到发光强度峰值。葡萄糖浓度在 1 .0× 1 0 -5～ 2 .0× 1 0 -2 mol/L范围内与发光强度呈线性关系 ,检出限为 6.4× 1 0 -6mol/L,可应用于市售饮料中葡萄糖的测定更多还原

【Abstract】 An optical fiber biosensor based on luminol electrochemiluminescence(ECL) was developed for determination of glucose. Using carbon paste as the solid support, glucose oxidase (GOD) was bulk immobilized in the carbon paste electrode. The enzymatic reaction electrooxidation of luminol and chemiluminescent reaction occurred simultaneously on the surface of the carbon paste electrode, so the response of the biosensor was very fast. The peak intensity was achieved within only 10 seconds after glucose injection. The linear range extends from 1.0×10 -5 to 2.0×10 -2 mol/L for glucose with detection limit of 6.4×10 -6 mol/L. The biosensor was applied to determination of glucose in soft drinks.更多还原