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氯化血红素作为过氧化物模拟酶与HRP的比较研究
Comparative Studies on Hemin as a Mimic of Peroxidase with Horseradish Peroxidase
【摘要】 用荧光光谱和紫外 -可见吸收光谱法研究了氯化血红素 ( hemin)催化过氧化氢与对羟基苯丙酸反应的动力学和反应产物 ,表明 hemin同时具有模拟过氧化物酶、过氧化氢酶的活性 ,在反应过程中 hemin自身被氧化分解。 hemin催化反应的产物除与天然的辣根过氧化物酶 ( HRP)催化的产物相同 (λex/λem=31 0 nm/40 6nm)外 ,还有激发峰在 2 5 8nm和 30 0 nm,发射峰在 360 nm的荧光物质。通过对产物光谱特性的比较 ,研究了 hemin和 HRP的特异性差异。该研究结果对进一步筛选和研究高特异性过氧化物模拟酶具有重要的指导意义
【Abstract】 The reaction kinetics and spectral characteristics of products of the hemin catalyzed oxidation of p hydroxyphenylpropionic acid with hydrogen peroxide were studied through fluorometry and spectrophotometry. Hemin was found to show peroxidase like, catalase like activities, and to be decomposed due to oxidation and dimerization. Besides the primary product with maximum emission at 406 nm when excited at 320 nm, the system has a fluorescent by product with an emission peak at 360 nm when excited at 258 or 300 nm. The difference in specificity of hemin and horseradish peroxidase was examined by comparing spectral characteristics of reaction product. The competitive relationship of the multiple roles of hemin in the system was discussed. The result is of importance in mimicry of peroxidase with high specificity.
【Key words】 Hemin; Specificity; Mimic of enzyme; Catalytic fluorogenic reaction; Horseradish peroxidase;
- 【文献出处】 分析科学学报 ,Journal of Analytical Science , 编辑部邮箱 ,2001年04期
- 【分类号】O657.39
- 【被引频次】24
- 【下载频次】601