【摘要】 报道了人尿中脱氧核糖核酸 (DNA)氧化损伤产物 8 羟基脱氧鸟苷的气相色谱分析方法。采用固相萃取和衍生化前处理步骤 ,用色谱保留值及气相色谱 质谱进行定性鉴定 ,在给定的色谱条件下 ,尿中的 8 羟基脱氧鸟苷能与其他杂质很好地分离 ,内标法定量。方法的线性范围为 0 .2～ 10 0 μg ,FID的检测限为 4 .5 6pg s。分析尿样的相对标准偏差小于 8.0 %更多还原

【Abstract】 A method for analysing 8 hydroxydeoxyguanosine (8OHdG) in human urine by solid phase extraction and derivatization gas chromatography is described. The experimental conditions, especially the optimization of derivatization, were systematically studied. The 8OHdG was identified by gas chromatography/mass spectrometry. The detection limit of this method is 4.56 pg/s, and the linear range is 0.2～100 μg. For the analysis of 8OHdG in human urine, the RSD is less than 8.0% ( n =5).更多还原