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椭圆小球藻(Chlorella ellipsoidea)对4种重金属的耐受性及富集

Tolerance of Chlorella ellipsoidea and Its Removal of Heavy Metals

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【作者】 浩云涛李建宏潘欣马宇翔王雪锋

【Author】 HAO Yuntao LI Jianhong PAN Xin MA Yuxiang WANG Xuefeng (Department of Biology, Nanjing Normal University, Nanjing 210097, P.R.China)

【机构】 南京师范大学生命科学学院!南京210097

【摘要】 分离筛选获得了一株高重金属抗性的椭圆小球藻 (Chlorellaellipsoidea) ,并研究了不同浓度的重金属铜、锌、镍、镉对该藻生长和叶绿素a含量的影响及其对重金属离子的吸收富集作用 .结果显示 ,该藻对Zn2 +和Cd2 +具有很高的耐受性 .对四种重金属的耐受能力依次为锌 >镉>镍 >铜 .其叶绿素a含量与重金属离子浓度呈明显负相关 .该藻对重金属具有很好的去除效果 ,经 15μmol/LCu2 +、30 0 μmol/LZn2 +、10 0 μmol/LNi2 +、30 μmol/LCd2 +浓度 72h处理 ,去除率分别达到 4 0 .93%、98.33%、97.6 2 %、86 .88% ,可应用于含重金属废水的处理

【Abstract】 A strain of Chlorella ellipsoidea with high tolerance to heavy metals was isolated. Growth and heavy metals removal were studied on this alga exposed to different heavy metal concentrations. The effect of heavy metal on growth indicated that cells can live in higher Zn 2+ and Cd 2+ concentrations. The tolerant sequence to 4 kinds of metals for C. ellipsoidea was Zn 2+ >Cd 2+ >Ni 2+ >Cu 2+ . The content of chlorophyll a decreased with the increase of heavy metal concentrations. In this experiment, C. ellipsoidea also showed its rather strong ability to remove heavy metals from the medium. After 72-hour treatment, the removal rates of metals were 40.93%, 98.33%, 97.62% and 86.88% respectively, for 15 μmol/L Cu 2+ , 300μmol/L Zn 2+ , 100μmol/L Ni 2+ and 30μmol/L Cd 2+ . These results suggested that the C. ellipsoidea strain could be applied to the treatment of heavy metal wastewater.

【关键词】 椭圆小球藻重金属耐受性富集
【Key words】 Chlorella ellipsoideaheavy metaltoleranceremoval
【基金】 江苏省教委基金资助项目 (98KJD180 0 0 2 )
  • 【文献出处】 湖泊科学 ,Journal of Lake Science , 编辑部邮箱 ,2001年02期
  • 【分类号】X173
  • 【被引频次】127
  • 【下载频次】856
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