【摘要】 应用随机扩增多态性 DNA(RAPD)分析方法 ,分别对 3组花椰菜自交不亲和系和自交系的基因组 DNA进行差异分析。筛选出 2 0个 10 bp随机引物进行 RAPD反应 ,扩增片段分子量在 0 .3～ 3kb之间。其中分别有 3、2、 3个引物在各组中扩增出差异片段 5、 4、 5条。结果表明 ,花椰菜自交不亲和系与自交系基因组 DNA之间存在明显差异 ,扩增出的差异片段与花椰菜自交不亲和性相关。初步探讨了花椰菜自交不亲和性的遗传机制及RAPD技术在自交不亲和系选育过程中的应用前景更多还原

【Abstract】 Genetic DNA difference among three varieties of self\|incompatible and self\|compatible cauliflowers were analyzed by using Random Amplified Polymorphic DNA(RAPD). The total of twenty screened 10 bp primers were used in RAPD analysis. The molecular weight of every amplified fragment was between 0 3 kb and 3 kb. There were five, four and five differential bands respectwely which amplified with three, two and three primers. The results showed that, there were significantly difference between two kinds of genomic DNA. The differential bands were related to cauliflower self\|incompatibility. The genetic mechanism of cauliflower self\|incompatibility and application of RAPD in breeding of self\|incompatibility cauliflower were preliminary discussed.更多还原