【摘要】 目的　研究不同浓度胰岛素生长因子 - 1(IGF - 1)对兔成骨细胞增殖的影响。方法　采用组织块细胞培养技术进行兔成骨细胞分离培养 ,取第 2代成骨细胞分别与 0 .1ng/ml、1ng/ml、10ng/ml、2 0ng/mlIGF - 1培养 ,培养 2 4、36小时后行四唑盐比色法检测细胞增殖情况。结果　IGF - 1与成骨细胞培养 2 4、36小时 ,经MTT法检测 ,不同浓度IGF - 1与对照组比较 ,有显著性差异 (P <0 .0 1)。IGF - 1浓度为 0 .1、1、10ng/m1,各组之间相比存在显著性差异 (P <0 .0 5 )。结论　IGF - 1对成骨细胞有明显促进增殖作用 ,在 0 .1- 10ng/m1范围 ,存在浓度依赖性。更多还原

【Abstract】 Objective To investigate and compare the effect of insulin-like growth factor-1 on proliferation of rabbit osteoblast in vitro. Methods Osteoblasts were obtained from rabbit costal bone by explant culture technology. The second subculture was exposed to insulin-like growth factor-1 at variant concentrations of 0,0.1,1,10and 20ng/ml for 24 and 36 hours and MTT assay was employed to observe the effects of insulin-like growth factor-1 on osteoblast proliferation. Results Osteoblasts exposed to insulin-like growth factor-l at variant concentrations of 0.1,1,10,20ng/ml were significantly different compared with the control ( P <0.01),and significant differences were found between every two groups when osteoblasts were exposed to insulin-like growth factor-l at range from 0.1 to 10ng/ml ( P <0.05).Conclusions Insulin-like growth factor-l could significantly elevate the proliferation of osteoblasts. At range from 0.1-10ng/ml, It depended on concentration. MTT assay is a good kind of method for determining the proliferation of osteoblasts.更多还原