【摘要】 目的　探讨哈尔滨地区汉族肺癌患者α1 抗胰蛋白酶 (α1 AT)遗传多态性和其含量的动态变化及意义。方法　采用聚丙烯酰胺等电聚焦电泳法对 99例肺癌及 749例正常人血清α1 AT的基因表型进行测定 ,单相免疫扩散法测定治疗前后的α1 AT含量。结果　肺癌和对照组均能检测到α1 AT的M1 M1 、M1 M2 、M2 M3 等常见亚型 ,两组均未发现α1 AT缺失型。两组间各表型及基因频率均无显著性差异 (P >0 .0 5 )。肺癌组α1 AT含量明显高于对照组 (P <0 .0 5 ) ,经抗炎或 /和化疗后α1 AT显著升高 (P <0 .0 5 )。结论　肺癌患者α1 AT的遗传多态性与正常人无明显差异 ,但动态观察α1 AT含量对炎症与肿瘤的鉴别、评估肿瘤的化疗效果及对化疗方案的制定和调整具有一定参考价值更多还原

【Abstract】 Objective To study the gene polymorphism and the dynamic changes of the level of α 1 antitrypsin(α 1 AT) in patients with lung cancer of Chinese Han race in Harbin and its clinical significance. Methods The phenotypes of α 1 AT from 99 patients with lung cancer and 749 normal persons were determined by polyacrylamide gel isoelectricfocusing electrophoresis. The levels of α 1 AT in serum were determined by monophonic immunodiffusion. Results The common subtypes, such as M 1M 1,M 1M 2,M 2M 3,etc, were positive and the deficiency subtype was negative in both of the two groups. There was no significant difference in phenotypes and gene frequencies between the two groups (P>0.05). The level of α 1 AT in patients with lung cancer were remarkably higher than that in normal group (P<0.05), and significantly increased after anti inflammatory or/and chemical treatment (P<0.05). Conclusion There is no significant difference in gene polymorphism of α 1 AT between the patients with lung cancer and normal persons, but the dynamic changes of α 1 AT are valuable to differentiate inflammation from cancer, estimate the efficacy of chemotherapy, and formulate and adjust the treatment scheme.更多还原