【摘要】 实验中发现 ,在培养基中加入适量 PEG可以显著提高杜仲胚乳愈伤组织分化频率。PEG这种促进分化的效果既与 PEG的分子量和所用的浓度有关 ,也与培养基中无机离子的强度和蔗糖浓度有关。效果最佳的培养基配方是 :在激素组成为 BA(2 .0～ 2 .75 mg/L) +NAA 0 .1 5 mg/L)的基本培养基 (MS无机盐 +B5有机物 +3 %蔗糖 )中 ,添加浓度为 4%～6 %PEG40 0 0或 4%～ 5 %PEG6 0 0 0。在这种培养基上杜仲胚乳愈伤组织的分化频率均超过 5 0 %,最高可达 70 %以上 ,而在同样的条件下不加 PEG时分化频率不到 1 0 %。然而 ,经PEG处理分化出来的胚乳再生植株中 ,部分苗玻璃化现象严重更多还原

【Abstract】 It was found in the present study that redifferentiation frequency of callus in E.ulmoides could be raised remarkably by adding an appropriate concentration of PEG in the basal medium.In doing so,whats worth particular attention was as follows:the effect of PEG on promoting shoot redifferentiation was closely related to the molecular weight of PEG,and the concentration of PEG in basal medium.It was also related to the strength of inorganic ions,and the concentration of sucrose in the medium.The most effective recipe of basal medium was MS inorganic salt+B 5 organic supplements+3% sucrose,with phytohormones being BA (2.0~2.75 mg/L)+NAA (0.15 mg/L) and with 4%~6% PEG 4 000 or 4%~5% PEG 6 000 added in.In such a medium,over 50% of shoot redifferentiation frequency was achieved,while under the same condition except for the absence of PEG,the frequency turned out to be less than 10%.Unfortunately,many of shoots redifferentiation on the PEG containing medium suffered from vitrification,the solution to which is under our investigation.更多还原