【摘要】 根据其它植物 ACC合成酶 ( 1 - aminocyclopropane- 1 - carboxylic acid synthase,ACS)氨基酸保守区 ,设计 1组简并引物 ,用 RT- PCR法 ,从柿 ( Diospyros kaki Thunb.)果实扩增出3个约 1 kb左右的 c DNA片段 ,将其克隆至 p GEM- T载体上 ,对这些重组克隆进行序列测定和氨基酸序列推导 ,DK- ACS1由 1 1 0 1个碱基组成 ,编码 364个氨基酸 ;DK- ACS2是 1 0 86个碱基 ,编码 35 9个氨基酸 ;DK- ACS3为 1 0 89个碱基 ,编码 363个氨基酸。它们均具有其它植物 ACS合成酶中存在的 7个保守区和 1 1个不变氨基酸残基 ,且在多肽水平上有较高的同源性。与番茄 L E- ACS2的同源性 DK- ACS1是 60 .5 % ,DK- ACS2是 70 .7% ,DK- ACS3为66.9% ;与甜瓜 CM- ACS1的同源性依次分别是 60 .4% ,72 .1 %和 64.4%。更多还原

【Abstract】 According to the amino acid conserved region of ACC synthase (1-aminocyclopropane-1-carboxylic acid synthase,ACS) from other plant,a group of degeneracy primers were designed in this paper.Three cDNA fragments about 1 kb were amplified and cloned into pGEM-T vector from persimmon (Diospyros kaki Thunb.) fruit by RT-PCR.The result of sequencing and amino acid squence inference of these recombinant plasmid shows:DK-ACS1 has 1101 bases,encoding 364 amino acid,DK-ACS2 has 1086 bases,encoding 359 amino acid;and DK-ACS3 has 1098 bases,encoding 363 amino acid.They also have 7 conserved regions and 11 constant amino acid residues of ACC synthase gene that existing in other plant,and with higher identity in polypeptide level.The amino acid sequence of DK-ACS1,DK-ACS2 and DK-ACS3 are 60.5%,70.7% and 66.9% indential to LE-ACS2 from tomato;and are 60.4%,72.1% and 67.4% indentical to CM-ACS1 from melon,respectively.更多还原