【摘要】 不同浓度 ( 1～ 4μmol/ L APM)处理萌发的大麦、黑麦 2种种子 ,在 3μmol/ L APM下 ,大麦中诱导出 POD3(根系 )和 POD2 ,POD3(幼苗 )同工酶谱带 ,黑麦则在 4μmol/ L时诱导出 POD2 、POD4 (根系 )和 POD4 ,POD5(幼苗 )同工酶。蛋白质的变化则呈现出诱导出新的蛋白质或某些蛋白质谱带消失、活性减弱的特性。在 3～ 4μmol/ L APM浓度下诱导出某些新的蛋白质谱带 ( 31 k D,大麦幼苗 ;1 0 k D,大麦根系 ;66k D,8k D黑麦根系 )和某些谱带的消失或着色变浅 ( 88k D,大麦根系 ;66k D,大麦、黑麦幼苗 )。这一结果与 POD同工酶变化结果基本吻合 ,可以认为 3～ 4μmol/ L APM为上述作物的临界浓度。更多还原

【Abstract】 The changes in protein and isozymes of roots and seedlings in Hordeum vulgare、Scale cereale treated by APM have been studied.POD 3 in root,POD 2,POD 3 in seedling were induced by treatment of 3 μmol/L APM in H.vulgare .While POD 2,POD 4 in root and POD 4,POD 5 in seedling were induced under treatment by 4 μmol/L in S.cereale .Compared with control,some protein bands appeared while the some others disappeared and reduced in their activity.Treated with 3-4 μmol/L APM,31 kD protein of seedling in H.vulare ,10 kD protein of roots in H.vulgare and 66 kD,8 kD of root in S.cereale were induced,while 88 kD protein of root and 66 kD proteins in seedlings of the two plants faded away.It was almost the same as the changes in POD isozyme.So the 3-4 μmol/L APM was therefore considered the threshold concentration for the two plants.更多还原