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小麦——黑麦异代换及小麦种内易位系的分子细胞遗传学检测
Molecular cytogenetics detection of wheat-rye substitution and wheat intra-specific translocation line
【摘要】 研究应用基因组原位杂交、染色体 C-分带和 RAPD技术 ,对八倍体小黑麦×普通小麦杂种 F2 经电离辐射处理后的高代材料 98- 60进行了检测。基因组原位杂交结果表明 ,该材料为小麦 -黑麦异代换系。进一步通过 C-分带分析表明 ,该品系为 5R代换系 ,并且还包含有5AS/ 6AS小麦种内的染色体易位。通过 RAPD分析 ,在该品系中找到了来源于八倍体小黑麦亲本“新麦 73”的黑麦染色体特异扩增产物 OPA- 0 1 350 和与两个亲本不同的特异重组产物OPF- 1 480 0 、OPF- 1 492 0 ,进一步验证了基因组原位杂交和 C-分带的鉴定结果
【Abstract】 GISH,chromosome C banding and RAPD techniques were used in the detection of rye chromatin in high generation offspring (98 60) of radiation treated F 2 of crossing between octoploid triticale and common wheat.According to the result of GISH,these offspring are substitution lines,further analysis by C banding reveal the existence of 5R chromosome and a translocation of 5AS/6AS.These were also confirmed by RAPD that a amplified fragment,OPA 01 350 ,derives from octoploid triticale line “Xinmai 73” and two amplified recombination products,OPF 14 800 and OPF 14 920 ,are different from genomic DNA of two parents.
【Key words】 wheat； rye； GISH； C banding； RAPD； substitution； translocation；
- 【文献出处】 西北植物学报 ,Acta Botanica Boreali-occidentalia Sinica , 编辑部邮箱 ,2001年03期
- 【分类号】Q943
- 【被引频次】12
- 【下载频次】159