【摘要】 大果良种沙棘的幼嫩茎尖、茎段外植体接种在 MS、1 /2 MS附加不同浓度配比的IAA、IBA、BA、NAA培养基上可诱导茎尖及腋芽生长。将诱导产生的无性系芽接种在 MS或1 /2 MS附加 BA 0 .3～ 0 .5mg/L、NAA 0 .0 5mg/L的培养基上可形成丛生芽 ,同时在小叶片和嫩茎上诱导产生愈伤组织 ,继续培养愈伤组织表面形成大量的绿色突起 ,进一步分化成不定芽。在相同培养基上 ,不定芽上可直接产生不定芽 ,从而形成多达数百个的不定芽簇。不定芽长至 3cm时切下转至 1 /2 MS附加 IAA或 IBA0 .2 mg/L的培养基上可生根而形成完整的再生植株。更多还原

【Abstract】 Shoots and axillary buds were induced \%in vitro\% from stem apexes and stem segments of fine variety of Hippophae rhamnoides L.on MS and 1/2MS basic medium supplemented with different concentration of IAA,IBA,BA and NAA.When the regenerated buds obtained from the explant were inoculated on MS or 1/2MS basic medium containing 0.3-0.5 mg/L BA and 0.05 mg/L NAA,cluster buds were formed and calluses were induced from the leaflet and young stem.A large number of green prominencies were produced on callus surface in subculture process,and developed gradually to adventitious buds.Hundreds of adventitious buds might be produced on the surface of adventitious bud also on the same medium.When the 3 cm height adventitious bud was cut and transferred onto 1/2MS supplemented with IAA or IBA 0.2 mg/L,roots were induced and intact plants were regenerated.更多还原