【摘要】 目的 :探讨不同部位鳞癌的细胞角蛋白 ,增殖细胞核抗原基因产物表达的差异性 ,对研究肿瘤局部生长环境对肿瘤实质细胞的影响具有一定的理论意义。方法 :采用免疫组织化学LSAB法检测 69例不同部位鳞癌 (皮肤鳞癌 9例、鼻咽鳞癌 10例、喉鳞癌 12例、食管鳞癌 14例、宫颈鳞癌 12例、肺鳞癌 12例 )的细胞角蛋白 (cytokeratin ,CK)、增殖细胞核抗原 (prolireratingcellnuclearantigen ,PCNA)基因产物的表达情况。结果 :皮肤、食管鳞癌多为高分化鳞癌 ,鼻咽、肺鳞癌多为低分化鳞癌 ;CK在不同部位鳞癌的表达由强到弱依次为皮肤、食管、喉、宫颈、肺 ;CK阳性细胞在皮肤、食管鳞癌组织中由癌巢周边到中央表达逐渐增强 ,CK阳性细胞在鼻咽、肺鳞癌组织中散在分布 ;高分化鳞癌较低分化鳞癌表达强。PCNA的表达在鼻咽、肺鳞癌明显强于皮肤、食管鳞癌 (P <0 0 5)。PCNA阳性细胞在皮肤、食管鳞癌组织中主要位于癌巢周边 ;在喉鳞癌中位于癌巢的周边到中层 ;在鼻咽、宫颈、肺鳞癌中从癌巢周边到中央弥散分布。结论 :不同的生长环境可能对鳞癌的分化、增殖影响不同更多还原

【Abstract】 Objective:To investigate the different expression of CK and PCNA proteins of squamous cell carcinoma(SCC)in various tissues.Methods:The expression of CK and PCNA antigen proteins was examined by immunohistochemical staining in 69 cases SCC(9 skin,10 laryngeal,12 nasopharyngeal,14 esophageal,12 cervical and 12 lung SCC).Result:well differentiated SCC was mainly found in epidermal esophageal SCC,whereas poorly differentiated SCC was mainly found in nasopharyngeal and lung SCC.The strength of CK expression was as follows:skin,esophageal,laryngeal,cervical,nasopharyngeal and lung SCC.The number of CK proteins positive cells increased gradually in epidermal and esophageal SCC from the edge to the center of the cell nest,whereas diffused in nasopharyngeal and lung SCC.Significantly higher expression of PCNA proteins was found in nasopharyngeal and lung SCC compared with skin and esophageal SCC(P<0 05).PCNA positive cells were observed mainly on the edge of the cell nest in epidermal and esophageal SCC,whereas diffused in nasopharyngeal and lung SCC.Conclusion:These results suggest that enviroment may play an important role in differentiation and proliferation of squamous cell carcinoma.更多还原