【摘要】 研究了不同浓度的金属离子及有机化合物对枯草芽胞杆菌 (Bacillussubtilis )蛋白酶活力的影响 ,结果表明 :①在 5种金属离子中 ,Mg2 +、Na+、K+对酶有激活作用 ,其中Mg2 +浓度为 2mmol/L时 ,激活作用最大。随着金属离子浓度的降低 ,K+和Na+对酶的激活作用均减弱 ,Ca2 +和Zn2 +对酶活力有抑制作用。②在 7种重金属离子中 ,除Mn2 +对蛋白酶有激活作用外 ,Cu2 +、Pb2 +、Cd2 +、Co2 +、Cr3+和Cr6+均对蛋白酶有抑制作用。③在有机化合物中 ,半胱氨酸和EDTA对酶均有激活作用 ,且在 2 0mmol/L时最为强烈。④Na+-K+、Ca2 +-Mg2 +和Cu2 +-EDTA共同作用时对蛋白酶活力均有不同程度的抑制作用更多还原

【Abstract】 Effects of some ions and organic compounds on protease activities of Bacillus subtilis were investigated It was found that: ① Of 5 inorganic ions tested, Mg 2+ , Na + and K + were shown to activate the protease activities and Mg 2+ had the strongest effect on protease at 2 mmol/L The impacts of Na + and K + declined with decrease in concentrations, respectively. Zn 2+ and Ca 2+ inhibited the protease activities. ② All seven heavy metal ions were shown to have inhibition except Mn 2+ which activated the protease at three tested concentrations ③ Both cysteine and EDTA had the activated effects on protease activities and the effects were most remarkable at 20 mmol/L. ④The combinations of Na + and K + , Ca 2+ and Mg 2+ , EDTA and Cu 2+ all inhibited the protease activities to some extents更多还原