【摘要】 目的:以β-半乳糖苷酶基因为标志基因研究重组复制缺陷腺病毒在小鼠肺内气道上皮细胞中的转基因表达。方法:带有LacZ表达基因的重组复制缺陷腺病毒(AdCMVLacZ),经鼻腔吸入法和气管内注射法两种途径分别感染小鼠肺组织,此后在不同时间取小鼠肺组织经多聚甲醛固定后,以X-gal染色。石蜡包埋切片后用核固红复染。结果:β-半乳糖苷酶经重组复制缺陷腺病毒AdCMVLacZ感染小鼠气道和肺泡上皮细胞,并高效表达目的基因,表达时间可长达31天,重复感染后仍可表达13天。结论:重组复制缺陷腺病毒可携带外源性基因在小鼠气道上皮细胞内进行高效、稳定和较为长期的转基因表达。更多还原

【Abstract】 Objective: To investigate adenovirus - mediated tranagene expression in murine lung in vivo with LacZ gene as reporter gene. Methods: Recombinant duplication - deficient adenovirus vector with LacZ gene (AdCMVLacZ) administrated into murine lung by nose drop or instillation through trachea. Sacrifice mice at different time, then detect the activity of β-galactosidase by X-gal staining. Result: After transfection by AdCMVLacZ, the activity of β-galactosidase lasted for 31 days after the first adenoviral administration, and was still showed for 13 days after second adenoviral transfection. Conclusion: Rrecombinant duplication-deficient adenovirus vector can express extrogeneous gene in a high efficient, stable and relatively long period matter in murine lung epithelial cells in vivo.更多还原