【摘要】 目的 :用 TRAP- EL ISA法筛选表达端粒酶活性的消化道肿瘤细胞株 ,探讨端粒酶活性表达在消化道肿瘤中的意义 ,为进一步研究端粒酶特性提供可靠的方法学保证。 方法 :对 15株消化道肿瘤细胞株 (胃癌 4株、肝癌 6株、胰腺癌 2株、结肠癌 3株 )常规培养后 ,进行 TRAP- EL ISA检测 ,以正常肝细胞为对照。 结果 :15株肿瘤细胞中 ,除肝癌 BEL - 740 4和结肠癌L o Vo细胞株外 ,余 13株 (86 .7% )细胞均表达端粒酶活性 ,而正常肝细胞 L - 0 2不表达。 结论 :绝大多数消化道肿瘤细胞株表达端粒酶活性 ,提示端粒酶活性检测有潜在的临床应用价值。更多还原

【Abstract】 Objective: To select the telomerase positive cancer cell lines of gastrointestinal tract and to provide a convinced methodology for future telomerase study. Methods: Fifteen cancer cell lines (carcinoma of stomach 4, of liver 6, of pancreas 2, of colon 3) were cultured and telomerase activity were detected by TRAP ELISA. The normal hepatic cells were taken as control. Results: Thirteen cell lines were telomerase positive in the 15 lines(86.7%). Conclusion: Most of gastrointestinal tract cancer lines express telomerase, indicating the detection of telomerase activity has clinical potential. [更多还原