【摘要】 目的 :证实人胚胎脑及脊髓组织是否存在褪黑素 (Mel)受体 (MR) ,阐明 Mel生物学特性及对神经系统作用的机制。方法 :应用放射配体结合法检测人胚胎脑及脊髓组织 1 2 5 I- Mel特异结合情况。 结果 :视网膜 1 2 5 I- Mel特异结合量最高 ,视交叉及嗅球次之 ,其次为下丘脑、海马、脑干 ;动力学分析表明 Mel与其受体的结合为可逆性结合 ;特异性结合分析提示对 Mel呈高度特异性 ;10和 5 0μm ol/ L GTPγS使 1 2 5 I- Mel特异结合降低。 结论 :人胚胎脑和脊髓组织均存在 1 2 5 I- Mel特异结合位点 ;GTPγS对 1 2 5 I- Mel特异结合及亲和力均有一定影响 ,支持 MR与抑制性 G蛋白偶联的观点更多还原

【Abstract】 Objective: To verify whether there exists melatonin(Mel) receptor in human embryonic nervous system. Methods: Specific binding of Mel to embryonic brain and spinal cord was measured by radioligand binding assay. Results: 125 I Mel binding sites in optomeninx was the most, in eptochiasm and sniff ball was next; GTPγS dose dependently inhibited the binding. Conclusion: The results demonstrate the presence of specific binding of Mel in human embryonic brain and spinal cord. GTPγS has some effect on 125 I Mel specific binding,supporting the theory that Mel receptor is coupled to inhibitory G proteins.更多还原