【摘要】 以来自不同地点的红皮云杉胚乳为材料提取ＤＮＡ研究了ＤＮＡ提取的最佳时段．从而为构建红皮云杉的遗传图谱做准备．结果表明：提取ＤＮＡ样品的最佳时段是在种子生根的晚期、即根长到４ｃｍ以后．提取的 ＤＮＡ样品纯度和浓度都较高；单一胚乳中ＤＮＡ量完全能够满足大量 ＰＣＲ扩增的需要。更多还原

【Abstract】 In this paper. it mainly approaches the best time to extract DNA from endosperms of Picea koraiensis Nabal. which will be the starting work of making the genetic map for the conifer species in the future. The results are shown as follows: it is feasible to extract total DNA from single endosperm; the best time to extract DNA is in the late time of seed germination. with the root in 4cm length or more. when the nutrition in seed lins been used out. and also the amount and concentration of extracted DNA are all higher than that in the early time. Although one endosperm is small. extracted DNA is enough to meet PCR amplification reactions.更多还原