【摘要】 茉莉花芳樟醇 β_D吡喃葡萄糖苷酶 （LGA）丙酮粉经过pH6 0柠檬酸缓冲液萃取、 3 0 %和 80 %硫酸铵分步沉淀 ,以及CM_Toyopearl65 0M柱层析 ,用 0 - 0 5MNaCl柠檬酸缓冲液梯度洗脱酶蛋白 ,最后用快速蛋白液相色谱 （FPLC）的SephacrylS₂ 0 0柱分离系统纯化出了LGA ,LGA蛋白的SDS_PAGE图谱在分子量 1 4 4万道尔顿处显示了一条谱带。它可以催化芳樟基 β_D吡喃葡萄糖苷分解释放芳樟醇更多还原

【Abstract】 The acetone powder of linalyl β_D pyranoglucosidase（LGA） from Jasminum sambic flower was homogenized with 0 1 M pH6 0 citrate buffer and centrifuged, the supernatant was filtrated by 0 2 μm membrane filtration, then make the fractional precipitation of crude enzyme solution with 30% and 80% （NH4） 2SO 4 After centrifugation, the precipitate was dissolved and dialyzed in the same buffer The dialyzed enzyme was added on CM_Toyopearl 650 M column and eluted by a linear gradient of 0-0 5 M NaCl in the citrate buffer. The fraction containing LGA activity was collected, and freeze dried and dialyzed, the dialyzed solution was placed on a Sephacryl S₂00 column and eluted by the same buffer. A part of fraction containing LGA activity was assayed by SDS_PAGE with CBB staining method, the result of electrophoresis showed one band on the gel, the molecular weight （WM） of this protein was about 14 4kD.The other part containing LGA activity showed two bands, MW was 96 0kD and 30 0kD respectively.更多还原