节点文献
铜锌超氧化物歧化酶与丝氨酸钴(Ⅱ)的相互作用
Study on the Interaction of Copper-Zinc Superoxide Dismutase with Cobalt(Ⅱ)-Serine by Spectral Analysis
【Abstract】 The effect of changing the amount of externally added cobalt(Ⅱ) serine, an organic metal compound, and the pH value of the enzyme solution on the interaction of copper zinc superoxide dismutase (Cu 2Zn 2SOD) with cobalt(Ⅱ) serine was studied by means of visible spectroscopy, inductively coupled plasma atomic emission spectrometry and measurement of enzyme activity. It was found that in aqueous solution, there exists a direct interaction of the metal ions of the active center in Cu 2Zn 2SOD with cobalt(Ⅱ) serine. As a result, part of the metal ions in the metalloenzyme were replaced by Co ion to form Co(Ⅱ) substituted derivatives of SOD and the catalytic activity of the enzyme decreased.
【关键词】 铜离子；
锌离子；
超氧化物歧化酶；
丝氨酸；
钴；
相互作用；
【Key words】 copper ion； zinc ion； superoxide dismutase； serine； cobalt； interaction；
【Key words】 copper ion； zinc ion； superoxide dismutase； serine； cobalt； interaction；
【基金】 国家自然科学基金 ( 2 0 1710 0 9);大连大学科学基金资助项目
- 【文献出处】 催化学报 ,Chinese Journal of Catalysis , 编辑部邮箱 ,2001年06期
- 【分类号】O643.3
- 【被引频次】1
- 【下载频次】87