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溶菌酶在硅烷化石英表面吸附的压电传感监测

Monitor the adsorption of lysozyme on methylsilaned quartz surface by a piezoelectric sensor

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【作者】 康琪李敏马小隆李文鹏申大忠

【Author】 KANG Qi 1, LI Min 1, MA Xiao long 1, LI Wen peng 2, SHEN Da zhong 2 (Dept. of Chem. Engin., Shandong University of Science and Technology, Jinan, 250031 China; 2.Sch. of Chemistry and Chem. Engin., Shandong University,Jinan,250100 China)

【机构】 山东科技大学化工系山东大学化学与化工学院山东大学化学与化工学院 山东济南250031山东济南250031山东济南250100山东济南250100

【摘要】 石英表面经硅烷修饰后 ,可通过憎水性相互作用吸附溶菌酶 ,该吸附动力学过程以压电传感器实时监测。结果表明溶菌酶以单分子层吸附 ,吸附仅部分可逆 ,吸附和解吸的速率常数分别为ka =36 .4mol-1.kg .s-1,kd =8.92 × 10 -3 s-1,且可逆吸附比及饱和单层的吸附密度随溶菌酶浓度增加而增加

【Abstract】 Quartz surface that was modified by dichchlorodimethylsilane can adsorb lysozyme by the hydrophobic interaction. This adsorption kinetics process was monitored real time by a piezoelectric sensor. It was shown that lysozyme was adsorbed in monolayer and partly reversible. The rate constants for adsorption and desorption were estimated to be k a=36.4mol -1 .kg.s -1 and k d=8.92×10 -3 s -1 ,respectively.The ratio for reversible adsorption and the adsorption densities of saturation monolayer increases with increasing lysozyme concentration.

【基金】 山东省自然科学基金项目 (Q99B0 1)
  • 【文献出处】 传感器技术 ,Journal of Transducer Technology , 编辑部邮箱 ,2001年12期
  • 【分类号】TP212.1
  • 【被引频次】2
  • 【下载频次】54
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