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溶菌酶在硅烷化石英表面吸附的压电传感监测
Monitor the adsorption of lysozyme on methylsilaned quartz surface by a piezoelectric sensor
【摘要】 石英表面经硅烷修饰后 ,可通过憎水性相互作用吸附溶菌酶 ,该吸附动力学过程以压电传感器实时监测。结果表明溶菌酶以单分子层吸附 ,吸附仅部分可逆 ,吸附和解吸的速率常数分别为ka =36 .4mol-1.kg .s-1,kd =8.92 × 10 -3 s-1,且可逆吸附比及饱和单层的吸附密度随溶菌酶浓度增加而增加
【Abstract】 Quartz surface that was modified by dichchlorodimethylsilane can adsorb lysozyme by the hydrophobic interaction. This adsorption kinetics process was monitored real time by a piezoelectric sensor. It was shown that lysozyme was adsorbed in monolayer and partly reversible. The rate constants for adsorption and desorption were estimated to be k a=36.4mol -1 .kg.s -1 and k d=8.92×10 -3 s -1 ,respectively.The ratio for reversible adsorption and the adsorption densities of saturation monolayer increases with increasing lysozyme concentration.
【Key words】 adsorption; lysozyme; electrode separated piezoelectric sensor;
- 【文献出处】 传感器技术 ,Journal of Transducer Technology , 编辑部邮箱 ,2001年12期
- 【分类号】TP212.1
- 【被引频次】2
- 【下载频次】54