【摘要】 目的 :研究不同的缺血时间再灌注 0 .5 h对脑缺血组织的损伤程度及乙酰胆碱酯酶 ( ACh E)的影响。方法 :建立大鼠局灶性脑缺血动物模型 ,制备并纯化抗神经系统 ACh E的单克隆抗体 2 E6,观察缺血再灌注脑组织的病理变化及 ACh E含量。结果 :不同缺血时间再灌注 ,脑组织的损伤程度不同 ,ACh E的变化亦不同。结论 :单克隆抗体 2 E6可以用于神经系统 ACh E含量的检测 ,ACh E可能与缺血再灌注损伤有关更多还原

【Abstract】 Objective:To study the effects of reperfusion for 0 5 h after different ischemic duration on injury degree and acetylcholinesterase(AChE) level.Methods:Model of focal ischemia of brain ischemia reperfusion was established in rats,monoclonal antibody 2E6 to nervous systems AChE was prepared and purified.The pathological injury and AChE level in brain after ischemia reperfusion were observed.Results:Reperfusion after ischemia for different time could cause differen degree of injury and induce different level of AChE expression.Conclusion:Monoclonal antibody 2E6 could be used for measurement of AChE level in nervous system which might be related to ischemia reperfussion.更多还原