【摘要】 目的 :研究肝脂素 (YS2 H)对人白血病细胞及基质细胞生长的影响。方法 :采用白血病祖细胞集落生长方法、3H- Td R掺入法 ,观察 YS2 H对 2 5例急性髓细胞白血病患者的骨髓白血病细胞作用 ;采用骨髓基质细胞培养法观察经 YS2 H作用 ,培养 4周时的正常人及白血病患者骨髓基质细胞形态变化。结果 :YS2 H对体外人的急性髓细胞白血病细胞有明显的生长抑制作用 ,存在剂量依赖关系 ,而对正常骨髓 CFU- GM集落形成影响不大 (P>0 .0 5 ) ;同时发现 ,YS2 H可使基质细胞悬浮液中的白血病细胞及集落数减少 ,并使白血病的骨髓基质细胞形态趋向正常骨髓基质细胞形态。结论 :YS2 H是一种有选择性抑制白血病细胞生长的脂类抗白血病药物 ,具有临床研究应用价值。更多还原

【Abstract】 Objective:To study the effect of Heplipin(YS2H)on leukemia marrow and stromal cell in vitro.Methods:The effect of YS2H on bone marrow cells from 25 patients of AML with 3H TdR and colony assay were.The morphology of healthy persons and leukemia patients of bone marrow stromal cells with bone marrow stromal cells cultivation by YS2H were studied after 4 weeks.Rusults:Suppression ration of Heplipin to AML cells was,dose dependent (90 % in 300 mg/L);colony numbers of normal bone marrow CFU GM cells were not influenced by Heplipin.Configuration of stromal cells restored according to decrease of leukemia cells by Heplipin.Conclusion:Heplipin can be expected as a good anti leukemia lipid remedy in the aspect of the very mild side effect and strengthening normal bone marrow cells,there is clinical value of investigation and use.更多还原